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KMID : 0382420010270040035
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Korean Journal of Environmental Health Society
2001 Volume.27 No. 4 p.35 ~ p.40
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of Deoxynivalenol Producing Strains from Agricultural Products by Immunoanalytical Method
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Kang Sung-Jo
Oh Sang-Suk
Chung Duck-Hwa
Kim Hyoung-Kab
Park Jung-Hyun
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Abstract
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In order to evaluate the safety of agricultural products in Korea, we carried out work by screening of Fusarium species, which can produce deoxinivalenol(DON) from agricultural products in Western Gyeongnam, Korea. From 215 samples of soil and agricultural products, 129 strains of Fusarium species were obtained. The isolated strains were cultured at 28℃ for 14 days in rice mediums, and then extracted with 84% acetonitrile. The production of DON was verified by thin layer chromatography(TLC). As the results of TLC, 25 strains were identified as DON producing strain. But, only 10 strains were identified as DON producing strains by enzyme linked immunosorbent assay(ELISA). The levels of DON production were shown from 20 to 90㎍/g of rice medium. The maximum DON producing strain No. 41 was isolated from corn. In conclusion, the above results indicate that DON producing fungi contaminated agricultural products in Korea.
Therefore, further studies are required to accumulate more detailed data about the contamination of DON in various agricultural products.
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KEYWORD
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